The bacteria were capable to grow on various hydrocarbon like diesel, petrol, lubricating oil, toluene, naphthalene and kerosene. Addition of nitrogen and phosphorus sources was investigated for the growth of petroleum hydrocarbondegrading bacteria. The aim of this study was isolation and characterization of new marine oildegrading bacteria from a seawater sample. Biosurfactants and oil bioremediation eliora z ron and eugene rosenberg. Petroleum hydrocarbondegrading bacteria for the remediation of. Isolation and identification of diesel oildegrading bacteria in used. Isolation and identification of hydrocarbon degrading. Isolation and characterization of two crude oildegrading fungi strains from rumaila oil field, iraq. The strains were able to degrade about 90% of 100 mgl phenol within 80 h as sole carbon and energy source.
A new marine oildegrading consortium ctod was isolated from constanta seawater using 5% vv fuel oil, as the sole carbon source. Study on the biodegradation of crude oil by free and. Bacterial biodegradation of crude oil using local isolates. Pdf bacterial biodegradation of crude oil using local. Assessing biodegradation of oil with marine bacteria.
Download screening and identification of diesel oil degrading. Similarly, in preliminary study 57 oil degrading bacteria were isolated from oil impregnated soil in search of potent hydrocarbon degrader 14. Author links open overlay panel mehdi hassanshahian a giti emtiazi b. Isolation and identification of diesel oildegrading bacteria in used engine oil contaminated. Ultrastructure of two oildegrading bacteria isolated from. For several years scientists have pursued genetic modifications to enhance the ability of various types of engineered bacteria to degrade oil spills, whether on land or in sea. Isolation and characterization of new marine oildegrading.
Deepsea oil plume enriches indigenous oildegrading bacteria. View enhanced pdf access article on wiley online library html view download pdf for offline viewing. Oildegrading bacterial populations were enumerated by the threetube mostprobablenumber mpn assay using 10fold serial dilutions of beach sand in growth medium. E3 was measured to evaluate whether these carriers would satisfy the requirement for biodegradation of oil spills. The effects of oildegrading bacteria on the environment. Because oildegrading bacteria can utilize only a limited group of hydrocarbons, bacteria attached and growing on an oil droplet become nutrientstarved once this group of hydrocarbons is depleted. Oil degrading bacteria are considered as the dominant hydrocarbon which helps in degrading the aquatic systems such as oceans. Anaerobic hydrocarbondegrading sulfatereducing bacteria. Screening and identification of diesel oil degrading. If you previously purchased this article, log in to readcube. Oil biodegradation and oildegrading microbial populations in marsh sediments impacted by oil from the deepwater horizon well blowout. Isolation, selection and biodegradation profile of phenol. Isolation, identification and characterization of oil degrading bacteria isolated 431 biochemical characterization of selected microorganisms. Isolation of kerosene degrading bacteria from soil samples.
Atlas and bartha, 1992 was transferred to each flask and sterilized. This study investigated the immobilizations with of bacteria two kinds of algal materials, enteromorpha residue and kelp residue. Polycyclic aromatic hydrocarbons pahs, as the major components of oil sludge, are hydrophobic and recalcitrant. With the sharp increase in population and modernization of society, environmental pollution resulting from petroleum hydrocarbons has. Periodically spilledoil input as a trigger to stimulate.
Several hydrocarbondegrading sulfatereducing bacteria were enriched or isolated from marine sediments, however, in situ active microorganisms were to. In the present study, altogether 32 morphologically distinct bacteria were isolated from crude oil contaminated soil and 18 different hydrocarbondegrading bacteria were number. Oil is a toxic substance that can wipe out an entire population of marine life. Proteobacteria that are closely related to known petroleum degraders. These bacterias are capable of diverse metabolic pathways which enable them to utilize most recalcitrant petroleum hydrocarbons that are not present in fungi.
Adeleke1,3,4 background high demand for petroleum products all over the world has led to an increase in crude. Our results help to show the diversity of p450 and alkb genes in prokaryotes, and to portray the geographic distribution of oil. Isolation and characterisation of crude oil sludge. An oxygenconstrained system of crude oil reservoir environment was constructed to stimulate the growth of indigenous microbes, such as petroleum hydrocarbon degrading bacteria. The bp oil spill was infamous for the oil contamination in the cold, deep portions of the gulf of mexico, but little attention is focused on the discharged natural gas. Characterization of crude oil degrading bacteria isolated. Recent studies have identified bacteria from more than 79 genera that are capable of degrading petroleum hydrocarbons tremblay et al.
The lipophilicity of them were compared by diesel absorption rates. Methane degrading bacteria are usually limited in terms of degrading oil and oil degraders are usually limited in terms of degrading gases. Oil degrading and heterotrophic bacteria composition in. Enumeration, enrichment, and isolation of oildegrading bacteria. Here, we report that the dispersed hydrocarbon plume stimulated deepsea indigenous. In the present study, an aerobic bacterial strains ocsa and ocs b were isolated from an oil contaminated soil. Using physiological and biochemical identification, electron microscopy, and 16s rdna sequence analysis, strain sq was identified as advenella kashmirensis,sy was identified as. At the bottom of the mariana trench, the deepest part of the earths oceans, scientists have discovered unique oileating bacteria.
Native crude oil degrading bacteria were isolated from different crude oil polluted soils. Microbial ecology of oil degrading porous mafiadoc. Four petroleumdegrading bacterial strains, 2tnnb, 6tbxcl, mvk25, and xck, were isolated from various oilcontaminated sites in vietnam. An oxygenconstrained system of crude oil reservoir environment was constructed to stimulate the growth of indigenous microbes, such as petroleum hydrocarbondegrading bacteria. In recent years the microbial biodegradation of pollutants is a sustainable ways to clean up. Introduction the biodegradation of petroleum and other hydrocarbons in the environment is a. The present study deals with the isolation and screening of petroleum hydrocarbon degrading bacteria from petroleum refinery effluent pre.
Isolation of hydrocarbon degrading bacteria from soils. In this experiment, 25 oil degrading bacteria strains were isolated from the beach near dalian bay. Assessing biodegradation of oil with marine bacteria by monitoring o 2 and co 2 concentrations online in a closed loop introduction in april 2010, the deepwaterhorizon dwh well explosion at the gulf of mexico caused the worst oil. Of the 20 isolates, ram03, ram06, ram, and ram17 were specifically chosen based on their relatively higher growth on salt medium amended with 4 % crude oil, emulsion index, surface tension, and degradation percentage. Isolation and characterization of hydrocarbon degrading. Hydrocarbondegrading bacteria and the bacterial community. Isolation and characterization of bacteria from refinery. It is a conglomerate of benzene, toluene, ethylbenzene, xylene, along with high percentage of aromatic hydrocarbons that are.
The strains were identified to be citrobacter freundi and proteus mirabilis according to morphological, physiological and biochemical characteristics. Two hundred and fifty millilitre erlenmeyer flasks containing 100 ml of sterile mineral salts medium, 1. Of the 1 crude oil degrading isolates 15 isolates were selected and cultivated in bh media with 1% crude oil as a sole carbon and energy source. Isolation, identification and characterization of oil. Study of oil degrading bacteria isolated from oil contaminated sites.
Single colonies obtained from count of cultivable bacteria grown in onr7a medium with bilge waste, were selected and purified by repeated streaking under identical growth conditions. Nwinyi, obinna 2011 enrichment and identification of askarel oil pcb blend degrading bacteria enriched from landfill sites in edo state, nigeria. Characteristic assessment of dieseldegrading bacteria immobilized on natural organic carriers in marine environment. The use of microorganisms in remediating environmental contaminants such as crude oil sludge has become a promising technique owing to its economy and the fact it is environmentally friendly.
Isolation and characterization of bacteria degrading n. All books are in clear copy here, and all files are secure so dont worry about it. Microbial enhanced oil recovery meor is a tertiary oil recovery method that utilizes microbes to enhance oil production. Kerosene is a mixture of hydrocarbons that is deployed in aviation fuels and contributes to extremely noxious pollution during spills. Bacteria were isolated by means of the enrichment culture technique liu et al. During the experiments all cultures showed growth of bacteria and thus oil degrading bacteria were available as a food source for the protozoa. The modified polyelectrolytemagnetite nanocoating was. An important way of enhancing the rate of pah desorption is to compost. Pdf extraction and characterization of oil degrading bacteria. Enrichment and isolation of crude oil degrading bacteria from some mussels collected from the persian gulf. The current study aims to isolate oil degrading bacteria from soil and to test the degrading.
Isolation and characterization of hydrocarbon degrading bacterial. The microbial ecology of any biodegradation system is the fundamental factor that. Directional culture of petroleum hydrocarbon degrading. Isolation and identification of petroleum hydrocarbon. Inclusion bodies were found innhexadecanegrown cells and were absent in succinategrown cells. Bacteria and fungi are the key agents of degradation, with bacteria assuming the dominant role in. Isolation and identification of high efficient oil. The contaminated soils were located in tehran and isfahan oil refineries, iran. Ultrastructure of two oildegrading bacteria isolated from the tropical. Despite its toxicity, a considerable fraction of petroleum oil entering marine systems is eliminated by the hydrocarbondegrading activities of microbial communities, in particular by a recently discovered group of specialists, the hydrocarbonoclastic bacteria hcb.
Read online screening and identification of diesel oil degrading. The proliferation of oil degrading microbe implies the possible presence of oil residues including those. A smart dress for oildegrading bacteria sciencedaily. Presence of opportunistic oildegrading microorganisms operating. Extraction and characterization of oil degrading bacteria. Pdf on jan 1, 20, amit pandey and others published isolation of oil degrading bacteria from oil contaminated soil and expression of oil degrading genes in non oil degrading bacteria find. The inocula used consisted of either 100 microlitres. Alcanivorax borkumensis was the first hcb to have its genome sequenced. Dispersants set the stage for oildegrading bacteria. Two oildegrading bacteria identified aspseudomonas aeruginosa andmicrococcus luteus were isolated from crudeoilpolluted soils in nigeria. Gene diversity of cyp153a and alkb alkane hydroxylases in. The biological effects and expected fate of the vast amount of oil in the gulf of mexico from the deepwater horizon blowout are unknown owing to the depth and magnitude of this event.
Sediments samples were collected once in a month for a period of twelve months from area surrounding ennore creek and screened for hydrocarbon degrading bacteria. Characteristic assessment of dieseldegrading bacteria. Oil biodegradation and oildegrading microbial populations. Isolation and characterization of biosurfactant producing. Pdf study of oil degrading bacteria isolated from oil. Enrichment and identification of askarel oil pcb blend. Two oildegrading bacteria identified aspseudomonas aeruginosa andmicrococcus luteus. Petroleum hydrocarbons can be degraded by microorganisms such as bacteria, fungi, yeast and microalgae 3, 4. Because oil degrading bacteria can utilize only a limited group of hydrocarbons, bacteria attached and growing on an oil droplet become nutrientstarved once this group of hydrocarbons is depleted. Frontiers petroleum hydrocarbondegrading bacteria for. There are the two main approaches to oil spill bioremediation. Pdf hydrocarbon degrading microorganisms were isolated from ten oil polluted areas in and around navi mumbai. Isolation and characterization of crudeoildegrading. A minimal, artificial seawater medium was prepared and dispensed according to the method of widdel and bak.
Unique oileating bacteria discovered in worlds deepest. This research was focused on the isolation and characterization of indigenous bacteria from a south sumatras oilfield which were able to. Isolation and characterization of crude oil degrading. Two bacteria, strains ibbct6 and ibbct7 were isolated from the ctod consortium.
Pdf microbial degradation of petroleum hydrocarbons. A total of twenty bacterial cultures were isolated from hydrocarbon contaminated soil. Isolation and identification of oil degrading bacteria. Isolation and characterization of crudeoildegrading bacteria from the persian gulf and the caspian sea. The organisms were grown onnhexadecane and sodium succinate and then examined for the presence of hydrocarbon inclusions. The aim of this work was to isolate and characterize bacteria from two crude oilcontaminated soils which were capable of producing biosurfactant and degrade oil. Addition of nitrogen and phosphorus sources was investigated for the growth of petroleum hydrocarbon degrading bacteria. Study on immobilization of marine oildegrading bacteria. Identification and characterisation of oil sludge degrading bacteria. Because oil spills can have a devastating impact on the environment and organisms, the processes that the hydrocarbon degrading bacteria carry out increase the health of all the organisms around them.
Although oildegrading bacteria brought by floating oil had a positive effect on the development of a microbial community at the beach, the indigenous microbial. Agriculture and biology journal of north america, 2 1. Biosurfactants and oil bioremediation eliora z ron and. Onr7a media were supplemented with sterile 1% vv crude oil arabian light crude oil as the sole carbon source. Isolation and characterization of oildegrading bacteria. Isolation and characterisation of crude oil sludge degrading bacteria linda u. Characterization of petroleumdegrading bacteria from oil. Various traits, such as hemolytic activity, oil resolving, oil spreading, and oil emulsifying capability were used to screen the isolates.
571 1380 1072 1566 51 560 691 1427 967 1142 1015 694 900 688 1068 1218 24 1359 83 1213 546 1253 247 918 456 67 1059 1212 398 1016 356 1010 819 1386 1349 755 506 133 26